Members

Lab head

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Professor Marcelle Machluf, Ph.D

Prof. Marcelle Machluf received her Ph.D. in Biotechnology Engineering from the Ben-Gurion University (Israel). Marcelle went on to complete a 5-year post-doctoral fellowship at the Harvard Medical School under the supervision of Prof. Anthony Atalla. Marcelle joined the Technion in 2001, and received tenure in 2007; in 2014, she was promoted to full professor. Prof. Machluf has authored 40 peer-reviewed journal papers, co-authored 15 papers and published over 100 conference papers. Her papers have appeared in leading generalized and specialized journals including Nature Biotechnology, Nano Letters, and Blood, and have been cited over 2,500 times, awarding her an h-index of 26 (Google Scholar). Prof. Machluf has supervised more than 40 graduate and post-doctoral students, from around the world, and is teaching four undergraduate and graduate courses in nano-encapsulation, tissue engineering, mammalian cell technologies, and bioprocessing. Prof. Machluf is a member of the TargetCaRe consortium, under Horizon 2020 Training Networks (H2020-MSCA-ITN-2014). Prof. Machluf is the head of a six-year collaboration between six groups from the Technion and two of Singapore’s leading universities (NTU and NUS) funded by the National Research Foundation of Singapore. Prof. Machluf is a member of the Minerva Programme Committee, under the Max-Planck Institute (Germany) and a member of the Tissue Engineering Journal editorial board. Prof. Machluf is the Head of the Technion’s graduate Interdisciplinary Program in Biotechnology, and was recently appointed as the Deputy Executive Vice President for Research For the Pre-clinical Research Authority of the Technion.

E-mail: machlufm@tx.technion.ac.il

Short C.V.

Google Scholar profile

PUBMED citations

PUBLICATIONS


Management


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Dr. Tomer Bronshtein, Ph.D

Dr. Tomer Bronshtein is the manager of Prof. Machluf’s lab and is concentrating all data analysis, academic writing, and exploitation efforts of the nano-ghosts technology. Tomer received his Ph.D. in Biotechnology Engineering from the Technion, under the supervision of Prof. Machluf. The pivotal concept of using nano-ghosts for drug delivery was the subject of Tomer’s dissertation, aimed for treating HIV/AIDS. Dr. Bronshtein undertook a 3-year post-doctoral position in the Nanyang Technological University (Singapore) under the supervision of leading material scientists Subbu Venkatraman and Freddy Boey. During his fellowship, Dr. Bronshtein focused on developing MSC-based cardiac restoration therapies. Dr. Bronshtein specializes in drug delivery and mathematical computer-aided modeling of biological systems for regenerative medicine. E-mail: bronshtn@tx.technion.ac.il


Dr. Limor Baruch. Ph.D.

Dr. Limor Baruch, Senior research fellow, co-lab manager and Tissue Engineering and cell-based therapy group leader. Limor received her Ph.D. in Biotechnology Engineering from the Technion, under the supervision of Prof. Machluf. Limor’s dissertation presented the development of two cell- microencapsulation platforms and their application for cancer treatment. In 2008, Dr. Baruch established the laboratory for crop quality at Kaiima Bio-Agritech Ltd., and managed it for six years. Dr. Baruch expertise includes the setup of laboratories and research fields, diverse analytical methodologies and scientific consultancy in addition to cell-based therapy, drug delivery systems and regenerative medicine. E-mail: baruchl@bfe.technion.ac.il


Dr. Efrat Goren, Lab microbiologist. E-mail: gorenaa@techunix.technion.ac.il (profile under construction)


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Ms. Inna Kovrigina, M.Sc, Lab technician. E-mail: inna.ko777@gmail.com (profile under construction)


Post & Graduate Students


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Dr. Beth Schoen, Ph.D

Dr. Beth Schoen is a Fulbright Postdoctoral Fellow and an American Association for University Women Postdoctoral Fellow at the lab for cancer drug delivery and cell based technologies in the faculty of biotechnology engineering at the Technion-Israel Institute of Technology. Dr. Schoen received her Ph.D from Michigan State University and her BSc from the University of Florida both in chemical engineering. Dr. Schoen is part of the nano-ghost team and focuses on optimizing the PEGylation of the device. Dr. Schoen also studies tissue engineering and develops novel biological scaffolds that could be used to treat myocardial infarction and other damaged organs. E-mail: schoen54@tx.technion.ac.il


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Yael Efraim, B.Sc, Ph.D student

Yael Efraim, B.Sc is currently a ph.D student and serves as a teaching assistant at the Faculty of Biotechnology and Food Engineering at the Technion- Israel Institute of Technology. Yael’s research is in the field of tissue engineering, where it combines scaffold design and in-vitro cell culture. Her work focuses on injectable biological scaffolds and induced pluripotent stem cells (IPSC) for minimally invasive cell delivery to treat Myocardial Infarction. E-mail: yael.efraim@gmail.com 


Limor Sertshuk

Limor Sertshuk-Kaneti, B.Sc, Ph.D student

Limor completed her BSc in the Faculty of Biotechnology and Food Engineering at the Technion, and is currently a PhD student and serves as a teaching assistant. Her research focuses on the development of a novel non-viral targeted system for DNA delivery to cancer. The system is based on vesicles produced from the cell membrane of mesenchymal stem cells (MSCs). The vesicle’s production procedure preservers the surface molecules of these primary cells and thus benefits and exploits their unique ability to efficiently target and interact with tumor cells as well as with the tumor’s blood vessels’ endothelial cells. In addition, MSCs are known to be hypo-immunogenic and can even suppress the immune response – meaning we can use MSCs from multiple sources. While being non-viral, this gene delivery system avoids issues like clinical safety, limitation on the transgene size, and challenging production procedures. In this research, different therapeutic protein encoding plasmids are encapsulated within the cell-derived-vesicles, and their transfection efficiency will be evaluated in vitro and in vivo. E-mail: limorser@tx.technion.ac.il


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Maskit Gvirtz, B.Sc, Ph.D student

Maskit Gvirtz with a B.Sc in Biomedical Engineering is a Ph.D student at Biotechnology and food engineering as well as a teaching assistant at the Faculty in the Technion- Israel Institute of Technology.  Her goal is to design and study a mechanical- electrical stimulation apparatus for extra cellular matrix (ECM) thick cardiac tissue. In order to do so, she designed a computer integrated system that stimulates a left ventricle decellularized tissue, using an inflating/deflating balloon for mechanical stimulation, and a computer made signal transferred to a set of electrodes as an electrical stimulation. The computer can control the rhythm, volume of the mechanical stimulation, electrical signal shape and the synchronization between the two stimulations. The next stage is to study the effect of the system on different types of cells such as mesenchymal stem cells, cardiomyocytes etc. In order to improve, cell confluents, cell penetration depth into the tissue, cell growth, tissue tensile strength, cell organization, and unity in electrical conductivity. Controlling different aspects of cell growth on cardiac tissue will promote research of tissue engineering, and eventually, replacing organs with a non immunogenic lab made heart. E-mail: smaskit@gmail.com


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Nitzan Letko, B.Sc, Ph.D student

Nitzan completed her B.Sc in the Faculty of Biotechnology and Food Engineering at the Technion, and is currently a PhD student. She focuses on a novel drug delivery system – termed Nano Ghosts (NG), which is based on nano-vesicles produced from the plasma membrane of human Mesenchymal Stem Cells (MSCs), known for their targeting abilities towards wounds and inflammation. As a target she uses models of inflammation, attempting to encapsulate therapeutic agents and test their delivery and effect both in vitro and in vivo. E-mail: nitzanletko@gmail.com


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Noa Cohen, B.Sc, Ph.D student

Noa completed her BSc in the Faculty of Biotechnology and Food Engineering at the Technion and is currently an MSc student. Her research focuses on a novel drug delivery system aimed to target brain cancer across the Blood-Brain Barrier (BBB) using nano-vesicles derived from Mesenchymal stem cells (MSCs) which are known for their targeting abilities towards wounds and inflammation and have been shown to migrate across the BBB. MSCs derived vesicles (nano-ghosts) may serve as an effective drug delivery system for targeting brain inflammation and cancer. E-mail: noacoh@gmail.com


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Natali Malkah Dayan, B.Sc, M.Sc student

Natali completed her B,Sc in the Faculty of Biotechnology and Food Engineering at the Technion and is currently an M.Sc student. Her research focuses on a novel drug delivery system, Nano-Ghosts (NGs), aimed to selectively target lung cancer metastases while delivering therapeutic drugs. The NGs are nano-vesicles produced from the plasma membrane of human mesenchymal stem cells (hMSCs), known for their natural targeting of multiple cancers and hypo-immunogenicity. Different anti-cancer agents are encapsulated within the NGs system, such as Cisplatin, and their therapeutic index will be evaluated in vitro and in vivo. E-mail: natalid24@gmail.com


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Hagit Shalom, B.Sc, M.Sc student

Hagit completed her B.Sc in the Faculty of Biotechnology and Food Engineering at the Technion, and is currently a M.Sc student and serves as a teaching assistant. Her research focuses on a novel drug delivery system, based on cell-derived nano-vesicles termed nano-ghosts (NG) which are produced from the membrane of human Mesenchymal Stem Cells (hMSCs). The NGs preserve the MSC’s unique targeting capabilities toward inflammation and cancer. Hagit’s main goal is to elucidate the mechanism of interactions between the NG and the cancer cells in order to improve targeting for treating primary, metastatic and drug-resistant cancers. Furthermore, in order to track NGs in vivo and use them for imaging, she aims to load the NGs with fluorescent dyes or MRI contrast agents which may reveal early metastases. E-mail: hagitosa24@gmail.com


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Alexandra Ereskovsky, B.Sc, M.Sc student

Alex completed her BSc in the Faculty of Biotechnology and Food Engineering at the Technion and is currently a M.Sc student. Her research focuses on encapsulation of hydrophobic drugs (such as Paclitaxel) for cancer treatment within nano-vesicles derived from Mesenchymal stem cells (MSCs) which are known for their targeting abilities towards inflammation and cancer. E-mail: sashy236@gmail.com


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Stasia Krishtul, B.Sc, M.Sc student

Stasia completed her BSc in the Faculty of Biotechnology and Food Engineering at the Technion and is currently an MSc student. Her research focuses on a novel encapsulation platform for diabetes therapy. In this platform, insulin secreting cells are entrapped within microcapsules based on natural pancreatic ECM hydrogel, which provides the cells a natural fibrous niche that supports their viability and biological functionality. E-mail: technionstasia@gmail.com


Tom Givaty

Tom Givaty, B.Sc, M.Sc student

Tom completed her BSc in the Faculty of Biotechnology and Food Engineering at the Technion, and is currently a M.Sc student. Her research focuses on the development of a novel targeted non-viral delivery system for RNA interference, which is based on nano-ghosts produced from the cell membrane of mesenchymal stem cells. RNAi molecules (siRNA, miRNA etc.) serve as promising candidates for treating challenging diseases such as cancer, inflammatory diseases and auto-immune disorders. E-mail: tomgivaty@gmail.com


Domenico D’atri, M.Sc., Ph.D student (profile under construction). E-mail: domenicodatri88@gmail.com


Undergraduate Students

  • Lital Fried, B.Sc student, Final year project student
  • Ofek Elul, B.Sc student, Lab assistant
  • Tzila Davidov, B.Sc student, Lab assistant